Thermo Fisher
Dionex UltiMate 3000 Liquid Chromatography
Provisional entry — research in progress
This page was generated automatically from cited public sources and hasn't completed the full research pass yet. Every specification shown carries its source; more detail is added as research completes.
The Dionex UltiMate 3000 is a high-performance liquid chromatography (HPLC) system designed for analytical HPLC analysis. The Dionex UltiMate 3000 system is modular, consisting of a solvent rack and pump configurations including SD, RS, and BM pumps.[1][2][3]
What it is
The Thermo Scientific Dionex UltiMate 3000 is a high-performance liquid chromatography (HPLC) system used for the separation, identification, and quantification of chemical components in complex mixtures. The UltiMate 3000 series encompasses a modular platform of pumps, solvent racks, and other components designed for analytical, semi-preparative, and nano-flow LC applications. The system is manufactured by Thermo Fisher Scientific under the Dionex brand.[1][2][3]
How it works
HPLC systems operate by pumping a liquid mobile phase through a column packed with stationary phase material, where sample components are separated based on their differential interactions with the stationary phase. The UltiMate 3000 series includes pumps such as the LPG-3400, which is a low-pressure gradient quaternary pump, and various other pump configurations supporting isocratic, binary, and gradient elution modes.[3]
The solvent rack (SR-3000) serves as a solvent organizer and provides a secure location for installation of the solvent reservoirs. The solvent rack saves valuable bench space when mounted on top of the UltiMate 3000 system. All parts in the flow path are made of PEEK, FEP, stainless steel, or titanium (for biocompatible systems) to provide optimal resistance to common HPLC solvents and buffers.[2]
The UltiMate 3000 pumps feature a vacuum degasser (on select models like LPG-3400 and SRD-3X00) to remove dissolved gases from the mobile phase, preventing bubble formation that can impair separation and detection. The system is controlled via Chromeleon software, which provides methods development, automated control, data acquisition, and compliance tools such as wellness, predictive performance, and diagnostics.[3]
Applications
HPLC systems are widely used in pharmaceutical analysis, environmental testing, food safety, clinical diagnostics, and biochemical research. The UltiMate 3000 series supports analytical, semi-preparative, and nano-flow separations, including RSLCnano and Proteomics MDLC configurations for proteomics and biomarker discovery applications.[2][3]
What do the numbers mean?
Vacuum & pumping6
Gas & chemistry3
- Solvent reservoir capacity
- Eight 1-L, four 2.5-L, two 5-L, or combinations thereof, up to sixteen 0.5-L reservoirs[2]
Configuration & options8
Vintage & configurations
Documented models & variants
What does it need to run?
Site utility requirements, footprint, and infrastructure needed to install and operate this tool. Sourced from public records.
- Solvent reservoir capacityEight 1-L, four 2.5-L, two 5-L, or combinations thereof, up to sixteen 0.5-L reservoirs[2]
- Dimensions (solvent rack)10 x 42 x 51 cm (h x w x d)[2]
- Weight (solvent rack)3.0 kg without reservoirs[2]
- Pump typesSD, RS, BM[3]
- Typical purge flow rate (analytical pumps)3 mL/min[3]
- Typical purge flow rate (micro pumps)2 mL/min[3]
- Upper pressure limit (RS pumps)75 MPa[3]
- Upper pressure limit (SD pumps)45 MPa[3]
- Upper pressure limit (BM pumps)35 MPa[3]
Where are the manuals?
Generated from public-source data on file. Enter your email to access — nothing is published; details are routed privately.
Not publicly documented
Field notes
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Frequently asked questions
What types of pumps are available in the UltiMate 3000 series?
The UltiMate 3000 series includes SD, RS, and BM pump configurations such as ISO-3100 (isocratic), LPG-3400 (low-pressure gradient quaternary), DGP-3600 (dual gradient), HPG-3200 (high-pressure binary), and HPG-3400 (high-pressure quaternary). Biocompatible versions are available (e.g., ISO-3100BM, LPG-3400BM).[3]
What is the solvent reservoir capacity of the UltiMate 3000 solvent rack?
The solvent rack can accommodate: eight 1-L reservoirs, or four 2.5-L reservoirs, or two 5-L reservoirs, or five 1-L reservoirs and one 5-L reservoir, or three 2.5-L and two 1-L reservoirs.[2]
What materials are used in the flow path of the UltiMate 3000?
All parts in the flow path are made of PEEK, FEP, stainless steel, or titanium (biocompatible system only), providing resistance to common HPLC solvents and buffer solutions.[2]
How do I prime or purge the UltiMate 3000 pump?
Priming, also called purging, is performed by rinsing at a higher flow rate for a short time, manually or via autosampler. The pump can be purged from Chromeleon software or from the pump display by setting the channel to 100% and turning Purge to On. The purge cycle typically runs at 3 mL/min for analytical pumps (2 mL/min for micro pumps) for 5 minutes.[3]
What software controls the UltiMate 3000 system?
The system is controlled by Chromeleon software, which provides methods development, automated control, data acquisition, and features such as wellness, predictive performance, and diagnostics.[3]
Not publicly documented
The following facts about the Dionex UltiMate 3000 Liquid Chromatography are absent from this record as of this revision. First-hand knowledge or a citation closes a gap; every submission is reviewed before publication.
No publicly documented production dates or lifecycle milestones (introduction, end of production, EOL) for the Dionex UltiMate 3000 Liquid Chromatography are on record.
Answerable by: OEM historical records or a trade-press announcement
The control-system platform and OS era of the Dionex UltiMate 3000 Liquid Chromatography are not on record.
Answerable by: an engineer who operated it or OEM installation records
No publicly documented failure modes or field errata for the Dionex UltiMate 3000 Liquid Chromatography are on record.
Answerable by: a field service engineer, process engineer, or maintenance technician
The process node or technology generation of the Dionex UltiMate 3000 Liquid Chromatography is not on record.
Answerable by: an OEM datasheet or a fab qualification report
No publicly documented compatible parts, consumables, or accessories for the Dionex UltiMate 3000 Liquid Chromatography are on record.
Answerable by: an OEM parts catalog or a service engineer
No research found yet — worked with this tool? Share what you know.
Sources & citations
Sources (3)Every fact above is drawn from these public sources
- [1]inventory listing
- [2]manualslib.com — manualslib.commanualslib.com
- [3]manualslib.com — manualslib.commanualslib.com
Last updated Oct 1, 2026.
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